Articles, Blog

Cell Transport

September 19, 2019


Captions are on! Turn off by clicking “CC” at bottom right. Follow us on Twitter (@AmoebaSisters) and Facebook! Have you ever wondered what it must be like
to be inside a cell? Imagine the genetic material, the cytoplasm, the ribosomes—you will find
that in almost ALL cells—-prokaryotes and eukaryotes. Eukaryote cells in addition have
membrane bound organelles. All of those organelles have different functions. But cells are not
isolated little worlds. They have a lot going on inside them, but they also interact with
their environment. It makes sense that to keep a stable environment
inside them—otherwise known as homeostasis—they must have some control on what goes in and
out of them. A very important structure for this that ALL cells contain is the cell membrane.
By controlling what goes in and out, the cell membrane helps regulate homeostasis. Let’s take a look at the cell membrane.
You could have a course on the cell membrane itself—it has amazing structure and signaling
abilities. But to stick to very basics, it is made of a phospholipid bilayer. Bilayer
means two layers, so you have these two layers of lipids. Part of them—the head is polar.
The tail part is nonpolar. Some molecules have no problem going through
the cell membrane and directly go through the phospholipid bilayer. Very small non-polar
molecules fit in this category and are a great example. Like some gases. Oxygen and carbon
dioxide gas are great examples. This is known as simple diffusion. Also, it doesn’t take
any energy to force these molecules in or out so this is known as passive transport.
Simple diffusion moves with the flow. Meaning, it moves with the concentration gradient.
Molecules move from a high concentration to a low concentration. That’s the natural
way molecules like to move—from high to low—so when you hear someone saying it’s
going with the gradient then that’s what they mean. Remember how we said the cell membrane is
actually a pretty complex structure? Well, one thing we haven’t mentioned yet are proteins
in the membrane, and some of them are transport proteins. Some transport proteins act as channels.
Some of these proteins actually change their shape to get items across. Some of them open
and close based on a stimulus of some kind. And these are good things, because it’s
helping with molecules that may be too big to cross the membrane on their own or molecules
that are polar—and therefore need the help of a transport protein. This is known as facilitated
diffusion. It’s still diffusion, and it still moves with the concentration gradient
of high to low. It does not require energy so it is a type of passive tran sport. It’s
just that the proteins are facilitating, or helping, things pass. Charged ions often require
a protein channel in order to pass through. Glucose needs the help of a transport protein
to pass through. In osmosis, for water to travel at a fast rate across the membrane,
it passes through protein channels called aquaporins. These are all examples of facilitated
diffusion, which is a type of passive transport and moves with the concentration gradient
of high to low concentration. Now all the transport we’ve mentioned has
been passive in nature, that means it’s going from high concentration to low concentration.
But what if you want to go the other way? For example, the cells lining your gut need
to take in glucose. But what if the concentration of glucose in the cell is higher than the
environment? We need to get the glucose in and it’s going to have to be forced against
the regular gradient flow. Movement of molecules from low to high concentration takes energy
because that’s against the flow. Typically ATP energy. A reminder that ATP —adenosine
triphosphate—it has 3 phosphates. When the bond for the last phosphate is broken, it
releases a great amount of energy. It’s a pretty awesome little molecule. ATP can
power Active Transport to force those molecules to go against their concentration gradient,
and one way it can do that is actually energizing the transport protein itself. One of our favorite
examples of active transport is the sodium-potassium pump so that’s definitely something worth
checking out! –
There’s other times the cell needs to exert energy for transport – we’re still in
active transport for now. But let’s say a cell needs a very large molecule—let’s
say a big polysaccharide (if you check out our biomolecule video, that’s a large carbohydrate)—well
you may need the cell membrane to fuse with the molecules it’s taking in to bring it
inside. This is called Endocytosis— think endo for “in.” Often, this fusing of substances
with the cell membrane will form vesicles that can be taken inside the cell. Endocytosis
is a general term, but there are actual different types of endocytosis depending on how the
cell is bringing substances inside. Amoebas for example rely on a form of endocytosis. Pseudopods stretch out around
what they want to engulf and then it gets pulled into a vacuole. There are other forms
too such as the fancy receptor-mediated endocytosis—where cells can be very, very, very picky about
what’s coming in because the incoming substances actually have to bind to receptors to even
get in. Or pinocytosis—which allows the cell to take in fluids. So to the Google to
find out more details of the different types of endocytosis. Exocytosis is the reverse direction of endocytosis,
so this is when molecules exit—think exo and exit. Exocytosis can also be used to get
rid of cell waste but it’s also really important for getting important materials out that the
cell has made. Want a cool example? Thinking back to those polysaccharides—did you know
that large carbohydrates are also really important for making plant cell walls? Cell walls are
different from cell membranes—-all cells have membranes but not all cells have a wall.
But if you are going to make a cell wall, you’re going to need to get those carbohydrates
that are produced in the plant cell out of the cell to make the wall. So there’s a
great example of when you’d need exocytosis right there. Well that’s it for the amoeba sisters and
we remind you to stay curious! Follow us on Twitter (@AmoebaSisters) and Facebook!

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100 Comments

  • Reply Amoeba Sisters August 11, 2018 at 1:45 pm

    In case you're looking for more detail about the cell membrane itself—not just transport—we have a video called "Inside the Membrane" that you may want to check out: https://youtu.be/qBCVVszQQNs
    You can see our ENTIRE biology playlist here: https://www.youtube.com/playlist?list=PLwL0Myd7Dk1F0iQPGrjehze3eDpco1eVz

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  • Reply Hunter Bernier January 17, 2019 at 2:32 pm

    this was pretty good information

  • Reply TheCreepersAlive January 17, 2019 at 11:03 pm

    Does facilitated diffusion include channels like Aquaporin?? Or does facilitated diffusion have to involve transporter proteins,, because AQP is a channel not a transporter

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    did you talk about active transport?

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  • Reply David Villalba March 25, 2019 at 1:30 pm

    Cell transport have two things in common: endocytosis and exocytosis

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  • Reply Katie May 18, 2019 at 11:49 pm

    Can you do a vid specifically on Endo & Exocytosis. Like the steps?

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  • Reply Raya Kelley June 23, 2019 at 12:41 am

    gathering information from the Amoeba Sisters videos, exocytosis all while giving a thumbs up endocytosis and subscribing. This is made as easy as carbon and oxygen entering a cell membrane but thanks to their individuality this has avoided being as complicated as nitrogen and glucose travels to the main shindig. The animation explanations are as proteins that need a boost from the ends of our fingers producing views and possibly educated students, now we are, they are SUPER!! As solid as plant cell exocytosis!! Go us?

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  • Reply Carl Gemina September 1, 2019 at 1:38 pm

    why not mention bulk transport?

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  • Reply Nat M September 15, 2019 at 5:44 pm

    I’m in college Anatomy & Physiology and in 7 minutes this video explained way better than the 20+ pages of diffusion/active transport in my $120 textbook.

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